AgrobacTransV1, Main, Exploration, bibRecord, 000685

Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves.

Identifieur interne : 000685 ( Main/Exploration ); précédent : 000684; suivant : 000686

Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves.

Auteurs : Gisela Ferraro [Argentine] ; Melina Laguía Becher ; Sergio O. Angel ; Alicia Zelada ; Alejandro N. Mentaberry ; Marina Clemente

Source :

Experimental parasitology [ 1090-2449 ] ; 2008.

RBID : pubmed:18588877

Descripteurs français

English descriptors

KwdEn :
- Agrobacterium tumefaciens (MeSH), Animals (MeSH), Antigens, Protozoan (biosynthesis), Antigens, Protozoan (genetics), Antigens, Protozoan (immunology), Blotting, Western (MeSH), Caulimovirus (MeSH), Gene Expression Regulation, Plant (MeSH), Genetic Vectors (MeSH), Plant Leaves (genetics), Plant Leaves (metabolism), Protozoan Proteins (biosynthesis), Protozoan Proteins (genetics), Protozoan Proteins (immunology), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Tobacco (genetics), Tobacco (metabolism), Toxoplasma (genetics), Toxoplasma (immunology).
MESH :
- chemical , biosynthesis : Antigens, Protozoan, Protozoan Proteins, Recombinant Proteins.
- chemical , genetics : Antigens, Protozoan, Protozoan Proteins, Recombinant Proteins.
- chemical , immunology : Antigens, Protozoan, Protozoan Proteins, Recombinant Proteins.
- genetics : Plant Leaves, Tobacco, Toxoplasma.
- immunology : Toxoplasma.
- metabolism : Plant Leaves, Tobacco.
- Agrobacterium tumefaciens, Animals, Blotting, Western, Caulimovirus, Gene Expression Regulation, Plant, Genetic Vectors, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4(163-345)) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4(163-345) was placed under control of an additional PVX coat protein subgenomic promoter. In the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflower mosaic virus 35S promoter. His-Gra4(163-345) accumulation in agroinfiltrated tobacco leaves was estimated by Western blot analysis using mouse anti-Gra4 antibody and a seropositive human serum. Here, we demonstrated the feasibility of producing a Gra4 antigen using transient expression methods in plants.

DOI: 10.1016/j.exppara.2008.06.002
PubMed: 18588877

Affiliations:

Argentine

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Le document en format XML

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<author><name sortKey="Becher, Melina Laguia" sort="Becher, Melina Laguia" uniqKey="Becher M" first="Melina Laguía" last="Becher">Melina Laguía Becher</name>
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<author><name sortKey="Angel, Sergio O" sort="Angel, Sergio O" uniqKey="Angel S" first="Sergio O" last="Angel">Sergio O. Angel</name>
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<author><name sortKey="Zelada, Alicia" sort="Zelada, Alicia" uniqKey="Zelada A" first="Alicia" last="Zelada">Alicia Zelada</name>
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<author><name sortKey="Mentaberry, Alejandro N" sort="Mentaberry, Alejandro N" uniqKey="Mentaberry A" first="Alejandro N" last="Mentaberry">Alejandro N. Mentaberry</name>
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<author><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name>
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<author><name sortKey="Becher, Melina Laguia" sort="Becher, Melina Laguia" uniqKey="Becher M" first="Melina Laguía" last="Becher">Melina Laguía Becher</name>
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<author><name sortKey="Angel, Sergio O" sort="Angel, Sergio O" uniqKey="Angel S" first="Sergio O" last="Angel">Sergio O. Angel</name>
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<author><name sortKey="Zelada, Alicia" sort="Zelada, Alicia" uniqKey="Zelada A" first="Alicia" last="Zelada">Alicia Zelada</name>
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<author><name sortKey="Mentaberry, Alejandro N" sort="Mentaberry, Alejandro N" uniqKey="Mentaberry A" first="Alejandro N" last="Mentaberry">Alejandro N. Mentaberry</name>
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<author><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (MeSH)</term>
<term>Animals (MeSH)</term>
<term>Antigens, Protozoan (biosynthesis)</term>
<term>Antigens, Protozoan (genetics)</term>
<term>Antigens, Protozoan (immunology)</term>
<term>Blotting, Western (MeSH)</term>
<term>Caulimovirus (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (metabolism)</term>
<term>Protozoan Proteins (biosynthesis)</term>
<term>Protozoan Proteins (genetics)</term>
<term>Protozoan Proteins (immunology)</term>
<term>Recombinant Proteins (biosynthesis)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (metabolism)</term>
<term>Toxoplasma (genetics)</term>
<term>Toxoplasma (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Antigènes de protozoaire (biosynthèse)</term>
<term>Antigènes de protozoaire (génétique)</term>
<term>Antigènes de protozoaire (immunologie)</term>
<term>Caulimovirus (MeSH)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Protéines de protozoaire (biosynthèse)</term>
<term>Protéines de protozoaire (génétique)</term>
<term>Protéines de protozoaire (immunologie)</term>
<term>Protéines recombinantes (biosynthèse)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>RT-PCR (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Tabac (métabolisme)</term>
<term>Technique de Western (MeSH)</term>
<term>Toxoplasma (génétique)</term>
<term>Toxoplasma (immunologie)</term>
<term>Vecteurs génétiques (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antigens, Protozoan</term>
<term>Protozoan Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Protozoan</term>
<term>Protozoan Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Protozoan</term>
<term>Protozoan Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes de protozoaire</term>
<term>Protéines de protozoaire</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term>
<term>Tobacco</term>
<term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes de protozoaire</term>
<term>Feuilles de plante</term>
<term>Protéines de protozoaire</term>
<term>Protéines recombinantes</term>
<term>Tabac</term>
<term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes de protozoaire</term>
<term>Protéines de protozoaire</term>
<term>Protéines recombinantes</term>
<term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term>
<term>Tabac</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Animals</term>
<term>Blotting, Western</term>
<term>Caulimovirus</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genetic Vectors</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Agrobacterium tumefaciens</term>
<term>Animaux</term>
<term>Caulimovirus</term>
<term>RT-PCR</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Technique de Western</term>
<term>Vecteurs génétiques</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en">A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4(163-345)) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4(163-345) was placed under control of an additional PVX coat protein subgenomic promoter. In the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflower mosaic virus 35S promoter. His-Gra4(163-345) accumulation in agroinfiltrated tobacco leaves was estimated by Western blot analysis using mouse anti-Gra4 antibody and a seropositive human serum. Here, we demonstrated the feasibility of producing a Gra4 antigen using transient expression methods in plants.</div>
</front>
</TEI>
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<DateCompleted><Year>2008</Year>
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<Month>11</Month>
<Day>17</Day>
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<Title>Experimental parasitology</Title>
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<Abstract><AbstractText>A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4(163-345)) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4(163-345) was placed under control of an additional PVX coat protein subgenomic promoter. In the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflower mosaic virus 35S promoter. His-Gra4(163-345) accumulation in agroinfiltrated tobacco leaves was estimated by Western blot analysis using mouse anti-Gra4 antibody and a seropositive human serum. Here, we demonstrated the feasibility of producing a Gra4 antigen using transient expression methods in plants.</AbstractText>
</Abstract>
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<Author ValidYN="Y"><LastName>Zelada</LastName>
<ForeName>Alicia</ForeName>
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<Author ValidYN="Y"><LastName>Mentaberry</LastName>
<ForeName>Alejandro N</ForeName>
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<Author ValidYN="Y"><LastName>Clemente</LastName>
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Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves.

Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves.

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English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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